3JRA
Crystal structure of Fis bound to 27bp non consensus sequence DNA F6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.240, 95.220, 154.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.110 - 3.110 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.27800 |
| Structure solution method | 3IV5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.331 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.680 | 3.100 |
| Rmerge | 0.139 | 0.059 | 0.453 |
| Number of reflections | 11963 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 97.8 | 99.8 | 84.2 |
| Redundancy | 6.8 | 8.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 0.2 M Sodium citrate, 0.1 M TRIS-HCl pH 8.5, 36% PEG 400, vapor diffusion, hanging drop, temperature 277K |
