3J28
Dissecting the in vivo assembly of the 30S ribosomal subunit reveals the role of RimM
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
Method | Blot for 2 seconds before plunging |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 12.9 |
Software | MDFF,SPIDER |
Symmetry | C1 |