3GX8
Structural and biochemical characterization of yeast monothiol glutaredoxin Grx5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-10-29 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54178 |
Spacegroup name | H 3 |
Unit cell lengths | 85.120, 85.120, 48.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.214 - 1.673 |
R-factor | 0.1764 |
Rwork | 0.175 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yka |
RMSD bond length | 0.006 |
RMSD bond angle | 0.982 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.570 | 1.760 |
High resolution limit [Å] | 1.670 | 1.670 |
Rmerge | 0.034 | 0.303 |
Number of reflections | 15256 | |
<I/σ(I)> | 21.7 | 5.4 |
Completeness [%] | 98.5 | 89.6 |
Redundancy | 4.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 289 | 1.6M NH4SO4, Tris-HCl, pH8.0, VAPOR DIFFUSION, temperature 289K |