3GDW
Crystal structure of sigma-54 interaction domain protein from Enterococcus faecalis
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-31 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 43.127, 71.418, 94.502 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 50.000 - 2.000 |
| R因子 | 0.18503 |
| Rwork | 0.183 |
| R-free | 0.21431 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.329 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.020 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.109 | 0.606 |
| 独立反射数 | 20315 | |
| <I/σ(I)> | 65.7 | 6.35 |
| 完全性 [%] | 99.8 | 99.8 |
| 冗長性 | 13.7 | 13.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 297 | 0.1 M Sodium malonate, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
