3G7T

Crystal structure of dengue virus type 1 envelope protein in the postfusion conformation

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2007-07-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 63 2 2
格子定数 [Å]	75.650, 75.650, 292.802
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	25.000 - 3.500
R因子	0.212
Rwork	0.208
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ok8
結合長の平均二乗偏差(RMSD) [Å]	0.018
結合角の平均二乗偏差(RMSD) [度]	1.688
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.4.0077)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	3.630
分解能 [Å] (高)	3.500	3.500
Rmerge_l_obs	0.223	0.783
独立反射数	6794
<I/σ(I)>		1.64
完全性 [%]	98.7	92
冗長性	12.1	7.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	12.5% PEG 550 MME, 0.1 M MES pH 6.5, 10 mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	12.5% PEG 550 MME, 0.1 M MES pH 6.5, 10 mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	12.5% PEG 550 MME, 0.1 M MES pH 6.5, 10 mM ZnSO4, VAPOR DIFFUSION, HANGING DROP, temperature 289K