3FWZ
Crystal structure of TrkA-N domain of inner membrane protein ybaL from Escherichia coli
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-17 |
| Detector | ADSC QUANTUM Q315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 1 21 1 |
| 格子定数 [Å] | 34.801, 53.071, 72.537 |
| 格子定数 [度] | 90.00, 97.82, 90.00 |
精密化法
| 残基 | 50.000 - 1.790 |
| R因子 | 0.16968 |
| Rwork | 0.168 |
| R-free | 0.20159 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.470 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0054) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 1.820 |
| 分解能 [Å] (高) | 1.790 | 1.800 |
| Rmerge_l_obs | 0.063 | 0.196 |
| 独立反射数 | 24652 | |
| <I/σ(I)> | 47 | 11.65 |
| 完全性 [%] | 99.8 | 100 |
| 冗長性 | 4.9 | 4.4 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.2 M MgCl2, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
