3FBC
Crystal structure of the Mimivirus NDK N62L-R107G double mutant complexed with dTDP
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2007-09-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | C 2 2 21 |
| 格子定数 [Å] | 80.387, 153.337, 185.384 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 48.112 - 2.600 |
| R因子 | 0.192 |
| Rwork | 0.189 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b8p |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.008 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.186 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | PHENIX |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 48.200 | 2.740 |
| 分解能 [Å] (高) | 2.600 | 2.600 |
| 独立反射数 | 35512 | |
| <I/σ(I)> | 6.2 | 1.8 |
| 完全性 [%] | 98.8 | 98.9 |
| 冗長性 | 5.3 | 4.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40-44% MPD, 0.1M MOPS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
