3FBB

Crystal structure of the Mimivirus NDK N62L-R107G double mutant complexed with UDP

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	105
Detector technology	CCD
Collection date	2007-09-09
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	C 2 2 21
格子定数 [Å]	79.676, 152.899, 184.534
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	28.549 - 2.400
R因子	0.184
Rwork	0.181
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b8p
結合長の平均二乗偏差(RMSD) [Å]	0.009
結合角の平均二乗偏差(RMSD) [度]	1.272
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine))

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	70.000	2.530
分解能 [Å] (高)	2.400	2.400
独立反射数	44282
<I/σ(I)>	10.5	2.3
完全性 [%]	99.6	99.8
冗長性	6.7	6.8

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	40-44% MPD, 0.1M MOPS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K