3EWQ

HCov-229E Nsp3 ADRP domain

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798
Spacegroup name	P 21 21 21
格子定数 [Å]	47.820, 50.852, 68.278
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	30.000 - 2.100
R因子	0.20842
Rwork	0.204
R-free	0.28233
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.019
結合角の平均二乗偏差(RMSD) [度]	1.886
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	2.150
分解能 [Å] (高)	2.100	2.100
Rmerge_l_obs	0.234	0.234
独立反射数	9732
<I/σ(I)>		6.2
完全性 [%]	96.4	99.6
冗長性	12	12

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION	7.5	291	0.1M HEPES pH 7.5, 25% w/v PEG 3350, VAPOR DIFFUSION, temperature 291.0K