3ELH
X-ray structure of Acanthamoeba ployphaga mimivirus nucleoside diphosphate kinase complexed with dUDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.064 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 80.717, 153.576, 185.795 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.872 - 2.400 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b8q |
RMSD bond length | 0.006 |
RMSD bond angle | 1.088 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.700 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 43019 | |
<I/σ(I)> | 8.2 | 2 |
Completeness [%] | 99.3 | 99.6 |
Redundancy | 4.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 40 to 44% MPD, 0.1M MOPS, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |