3E79

Structure determination of the cancer-associated Mycoplasma hyorhinis protein Mh-p37

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2008-06-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
格子定数 [Å]	50.107, 69.149, 60.297
格子定数 [度]	90.00, 106.81, 90.00

精密化法

残基	50.000 - 1.900
R因子	0.189
R-free	0.24400
Structure solution method	SIRAS
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	0.021
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	SHELXL-97

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	50.000	1.970
分解能 [Å] (高)	1.900	1.900
独立反射数	29985
<I/σ(I)>	10.8	3.7
完全性 [%]	92.2	87
冗長性	4.1	4.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	Batch	3	298	0.1 M Ammonium Bromide, O.1 M Citric Acid, 40% PEG 4000, 9% N-decyl-beta-D-maltoside detergent. Crystal soaked in final concentration 0.7mM 5-amino-2,4,6-triiodoisophthalic acid, pH 3.0, Batch, temperature 298K