3CQ0
Crystal Structure of TAL2_YEAST
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-07 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.00 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 85.027, 113.461, 158.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.182 |
Rwork | 0.180 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f05 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.073 | 0.022 | 0.396 |
Number of reflections | 60407 | ||
<I/σ(I)> | 18.3 | ||
Completeness [%] | 99.4 | 95.2 | 99.1 |
Redundancy | 4.9 | 4.8 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 283 | 1.3M potassium citrate, 10% polyethylene glycol 200, 1% ethylene glycol, 0.1mM Hepes, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 283K |