3AIZ
Crystal structure of PCNA2-PCNA3 complex from Sulfolobus tokodaii (P21212)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-26 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 2 |
| 格子定数 [Å] | 91.110, 160.570, 116.560 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 48.640 - 2.800 |
| R因子 | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aix |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.002 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.472 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.5_2) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 49.095 | 2.950 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| 独立反射数 | 42314 | |
| <I/σ(I)> | 10.3 | 4.5 |
| 完全性 [%] | 98.9 | 97.6 |
| 冗長性 | 7 | 7.2 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.6M ammonium sulfate, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
