3A6R
E13Q mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-11-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.661, 83.583, 79.595 |
| Unit cell angles | 90.00, 94.72, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.200 |
| R-factor | 0.17145 |
| Rwork | 0.170 |
| R-free | 0.18945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.425 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.051 | 0.131 |
| Number of reflections | 140127 | |
| Completeness [%] | 94.4 | |
| Redundancy | 6.7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 24% PEG 6000, 0.1M Tris, 0.2M sodium acetate, 10% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
