3A3Q
Structure of N59D HEN EGG-WHITE LYSOZYME in complex with (GlcNAc)3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 281 |
Detector technology | IMAGE PLATE |
Collection date | 1995-06-19 |
Detector | MAC Science DIP-320 |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.710, 78.710, 38.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.21284 |
Rwork | 0.205 |
R-free | 0.28656 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lza |
RMSD bond length | 0.011 |
RMSD bond angle | 1.339 |
Data reduction software | PROTEIN |
Data scaling software | PROTEIN |
Phasing software | REFMAC (5.5.0088) |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.098 |
Number of reflections | 6611 |
Completeness [%] | 76.6 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 293 | 10% sodium chloride, 0.1M sodium acetate, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |