3A2G
Crystal Structure of K102C-Myoglobin conjugated with Fluorescein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-12 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1 |
Spacegroup name | P 6 |
Unit cell lengths | 90.409, 90.409, 45.320 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.610 - 1.750 |
R-factor | 0.17869 |
Rwork | 0.178 |
R-free | 0.19746 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2mbw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.348 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.092 | 0.304 |
Number of reflections | 21588 | |
<I/σ(I)> | 30 | 5.4 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 5.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 2.6M ammonium sulfate in 20mM Tris-HCl, pH9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |