2Z7E
Crystal structure of Aquifex aeolicus IscU with bound [2Fe-2S] cluster
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.7407, 1.7423, 1.6947, 1.5000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 72.903, 122.136, 62.051 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.250 - 2.300 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.25200 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.250 |
High resolution limit [Å] | 2.300 |
Number of reflections | 24440 |
Completeness [%] | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.6M ammonium sulfate, 0.5M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |