2Z1E
Crystal structure of HypE from Thermococcus kodakaraensis (outward form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.345, 45.833, 75.052 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.070 - 1.550 |
R-factor | 0.177 |
Rwork | 0.177 |
R-free | 0.20400 |
Structure solution method | MIRAS |
RMSD bond length | 0.012 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 44379 | |
<I/σ(I)> | 34.5 | 4.2 |
Completeness [%] | 98.5 | 89.2 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M acetic acid, 22%(w/v) MPD, 200mM NaCl, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |