2YQ2
Structure of BVDV1 envelope glycoprotein E2, pH8
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.180, 47.593, 159.290 |
Unit cell angles | 90.00, 108.15, 90.00 |
Refinement procedure
Resolution | 38.080 - 2.580 |
R-factor | 0.2406 |
Rwork | 0.240 |
R-free | 0.25270 |
Structure solution method | MAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.008 |
RMSD bond angle | 1.080 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX (AUTOSOL) |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.680 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.110 | 1.000 |
Number of reflections | 29885 | |
<I/σ(I)> | 27.6 | 1.7 |
Completeness [%] | 97.0 | 76.1 |
Redundancy | 5 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 52.5% METHYL-2,4-PENTANEDIOL (MPD) AND 0.1 M TRIS PH 8.0 |