2YPF
Structure of the AvrBs3-DNA complex provides new insights into the initial thymine-recognition mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.110, 100.250, 61.370 |
| Unit cell angles | 90.00, 102.55, 90.00 |
Refinement procedure
| Resolution | 32.290 - 2.550 |
| R-factor | 0.23857 |
| Rwork | 0.236 |
| R-free | 0.28983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v6t |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.897 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.290 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.040 | 0.620 |
| Number of reflections | 25764 | |
| <I/σ(I)> | 11.79 | 1.31 |
| Completeness [%] | 93.0 | 95.1 |
| Redundancy | 2.2 | 2.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M MES PH=6.5, 5-15% PEG3350, 5-15% 2-PROPANOL |
