2YHV
Structure of L1196M Mutant Anaplastic Lymphoma Kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2010-11-04 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.101, 57.760, 105.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.600 - 1.900 |
R-factor | 0.206 |
Rwork | 0.206 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yfx |
RMSD bond length | 0.004 |
RMSD bond angle | 0.800 |
Data reduction software | autoPROC |
Data scaling software | SCALA |
Phasing software | CNX |
Refinement software | CNS (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.190 | 2.000 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.060 | 0.470 |
Number of reflections | 25946 | |
<I/σ(I)> | 18.7 | 3.9 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.4 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.4 | 286 | CRYSTALS WERE GROWN AT 13 DEGREE CELSIUS BY VAPOR DIFFUSION AFTER MIXING 2 MICROLITERS OF PROTEIN SOLUTION WITH 2 MICROLITERS OF A RESERVOIR SOLUITON CONTAINING: 0.1 M TRIS PH 8.4, 0.2 M LITHIUM SULFATE, 0.1% (W/V) B-OCTYLGLUCOSIDE, AND 20% (W/V) PEG 3350. |