2YHT
Crystal structure of Hfq riboregulator from E. coli (P1 space group)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 |
Unit cell lengths | 61.200, 61.200, 53.100 |
Unit cell angles | 82.60, 87.30, 60.00 |
Refinement procedure
Resolution | 30.566 - 2.900 |
R-factor | 0.1833 |
Rwork | 0.181 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y90 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.154 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.080 | 0.140 |
Number of reflections | 14455 | |
<I/σ(I)> | 12 | 6.5 |
Completeness [%] | 97.4 | 82.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 293 | CRYSTALS WERE OBTAINED BY VAPOR DIFFUSION IN 2UL SITTING DROPS. THE RESERVOIR CONTAINED 30% PEG 3350, 0.1 M NA-CITRATE PH 5.4. CRYSTALLIZATIONS WERE CARRIED OUT AT 20 DEGREES CELSIUS. |