2XBU
Saccharomyces cerevisiae hypoxanthine-guanine phosphoribosyltransferase in complex with GMP (monoclinic crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-27 |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.577, 77.521, 56.655 |
Unit cell angles | 90.00, 95.13, 90.00 |
Refinement procedure
Resolution | 28.214 - 1.800 |
R-factor | 0.1689 |
Rwork | 0.167 |
R-free | 0.20580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jky |
RMSD bond length | 0.004 |
RMSD bond angle | 0.920 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.210 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.060 | 0.370 |
Number of reflections | 39543 | |
<I/σ(I)> | 15.3 | 3 |
Completeness [%] | 96.6 | 98.4 |
Redundancy | 4.3 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.2 M AMMONIUM ACETATE, 30% PEG 4000, 0.1 M TRI-SODIUM CITRATE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K, pH 5.6 |