2X79
Inward facing conformation of Mhp1
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 |
| 格子定数 [Å] | 173.888, 173.888, 74.502 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 33.388 - 3.800 |
| R因子 | 0.2773 |
| Rwork | 0.273 |
| R-free | 0.31260 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.115 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 40.000 | 3.940 |
| 分解能 [Å] (高) | 3.800 | 3.800 |
| Rmerge_l_obs | 0.080 | 0.690 |
| 独立反射数 | 47973 | |
| <I/σ(I)> | 17.6 | 1.4 |
| 完全性 [%] | 97.0 | 96 |
| 冗長性 | 3.8 | 2.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 9 | 24-28% PEG300, 100 MM NACL AND 100 MM BICINE PH9.0 |
