2WPD
The Mg.ADP inhibited state of the yeast F1c10 ATP synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-04 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 134.990, 173.944, 137.016 |
Unit cell angles | 90.00, 92.69, 90.00 |
Refinement procedure
Resolution | 39.775 - 3.432 |
R-factor | 0.2868 |
Rwork | 0.286 |
R-free | 0.29720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2HLD 1yce |
RMSD bond length | 0.004 |
RMSD bond angle | 0.629 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.800 | 3.620 |
High resolution limit [Å] | 3.430 | 3.430 |
Rmerge | 0.190 | 0.720 |
Number of reflections | 84424 | |
<I/σ(I)> | 4.3 | 2.3 |
Completeness [%] | 88.1 | 64.4 |
Redundancy | 2.8 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES/HCL PH 7.5, 12% PEG MME 5000, 100 MM SODIUM CHLORIDE MIXED 1:1 WITH PROTEIN SOLUTION CONTAINING 0.64 MM DDM, 25 MM TRIS/HCL PH 8.0, 100 MM SODIUM CHLORIDE, 25 MM TREHALOSE, 0.5 MM EDTA, 0.02% SODIUM AZIDE, 2 MM MAGNESIUM CHLORIDE, 0.66 MM ADP, 0.1 MM DCCD, 2.5 MM DTT, 0.5 MM PMSF |