2WAR

Hen Egg White Lysozyme E35Q chitopentaose complex

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 43 21 2
格子定数 [Å]	78.632, 78.632, 36.700
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	14.760 - 1.800
R因子	0.157
Rwork	0.155
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h6m
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.453
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.5.0082)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	15.000	1.860
分解能 [Å] (高)	1.800	1.800
Rmerge_l_obs	0.050	0.280
独立反射数	11116
<I/σ(I)>	40	6.4
完全性 [%]	93.0	95
冗長性	7	6.3

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		4.5		PROTEIN AT 7MG ML-1 IN 10MM CHITOPENTAOSE 200MM NA ACETATE PH5 WAS MIXED WITH RESERVOIR OF 200MM NA ACETATE, 4% W/V NACL PH4.5