2V88
Crystal structure of RAG2-PHD finger in complex with H3R2me2sK4me2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-10-06 |
Detector | RIGAKU-MSC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.200, 46.685, 56.869 |
Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.1958 |
Rwork | 0.196 |
R-free | 0.23160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v83 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.799 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.100 | 0.360 |
Number of reflections | 12678 | |
<I/σ(I)> | 16 | 2.9 |
Completeness [%] | 98.8 | 92.7 |
Redundancy | 4.7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | VAPOR DIFFUSION. HANGING DROP. 22% PEG 3350, 120-240 MM POTASSIUM FLUORIDE. TEMPERATURE 293K |