2V5M
Structural basis for Dscam isoform specificity
Experimental procedure
| 実験手法 | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 42 2 2 |
| 格子定数 [Å] | 99.172, 99.172, 163.991 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 20.000 - 1.950 |
| R因子 | 0.171 |
| Rwork | 0.169 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.014 |
| 結合角の平均二乗偏差(RMSD) [度] | 2.656 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.000 |
| 分解能 [Å] (高) | 1.950 | 1.950 |
| Rmerge_l_obs | 0.080 | 0.510 |
| 独立反射数 | 271422 | |
| <I/σ(I)> | 23.4 | 1.9 |
| 完全性 [%] | 97.8 | 91 |
| 冗長性 | 4.8 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 1.5 M AMMONIUM SULPHATE, 0.1 M HEPES PH 7.5 |
