2UP1
STRUCTURE OF UP1-TELOMERIC DNA COMPLEX
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | BRANDEIS |
| Spacegroup name | P 43 21 2 |
| 格子定数 [Å] | 51.200, 51.200, 171.090 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 47.700 - 2.100 |
| R因子 | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24900 |
| Structure solution method | MIRAS |
| Starting model (for MR) | 1up1 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.005 |
| 結合角の平均二乗偏差(RMSD) [度] | 23.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (0.3) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.070 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.051 | 0.218 |
| 独立反射数 | 14247 | |
| <I/σ(I)> | 14.2 | 10 |
| 完全性 [%] | 96.1 | 86.5 |
| 冗長性 | 3.8 | 2.6 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | Vapor diffusion, hanging drop * | 8.5 | pH 8.5 |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 3 | 1 | reservoir | glycerol | 15 (%) | |
| 4 | 1 | reservoir | 2.0 (M) |
