2RTB

APOSTREPTAVIDIN, PH 3.32, SPACE GROUP I222

Experimental procedure

Temperature [K]	273
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	I 2 2 2
Unit cell lengths	94.780, 105.320, 47.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.500 - 1.500
R-factor	0.209
Rwork	0.209
R-free	0.25800
RMSD bond length	0.018
RMSD bond angle	24.700 *
Data reduction software	bioteX ((MSC))
Data scaling software	bioteX
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.330 *
Rmerge	0.061
Total number of observations	78208 *
Number of reflections	34070
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *	20 *	Pahler, A., (1987) J. Biol. Chem., 262, 13933. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	potassium acetate	50 (mM)
3	1	drop		200 (mM)
4	1	reservoir	ammonium sulfate	30 (%sat)