2PGI
THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE-AN ENZYME WITH AUTOCRINE MOTILITY FACTOR ACTIVITY IN TUMOR CELLS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-25 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 75.100, 93.730, 171.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.25800 * |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 26.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.700 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.362 * |
| Total number of observations | 104060 * | |
| Number of reflections | 24024 | |
| <I/σ(I)> | 12 | 3.2 |
| Completeness [%] | 93.9 | 83.4 |
| Redundancy | 4.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | (mg/ml) | |
| 2 | 1 | drop | 6-phosphogluconate | (mM) | |
| 3 | 1 | drop | phosphate | (mM) | |
| 4 | 1 | reservoir | phosphate | (mM) | |
| 5 | 1 | reservoir | ammonium phosphate | (M) |
