2PC6
Crystal structure of putative acetolactate synthase- small subunit from Nitrosomonas europaea
Replaces: 2FGDExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97950 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 122.123, 122.123, 111.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.580 - 2.500 |
R-factor | 0.20846 |
Rwork | 0.205 |
R-free | 0.27573 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.838 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.580 | 2.570 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.084 | 0.431 |
Number of reflections | 28271 | |
<I/σ(I)> | 60.72 | 7.35 |
Completeness [%] | 99.9 | 100 |
Redundancy | 18.9 | 19.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2% TACSIMATE, 10% PEG 400, 0.1M KCl, 10mM CaCl2, 50 mM Na-HEPES, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |