2OPE
Crystal structure of the Neisseria meningitidis minor Type IV pilin, PilX, in space group P43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 98 |
Detector technology | CCD |
Collection date | 2005-05-18 |
Detector | BRUKER PROTEUM |
Spacegroup name | P 43 |
Unit cell lengths | 76.836, 76.836, 89.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.400 |
R-factor | 0.21156 |
Rwork | 0.208 |
R-free | 0.27893 |
Structure solution method | Combination of SAD and Mol. Replacement |
RMSD bond length | 0.018 |
RMSD bond angle | 1.486 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (for sad phasing) |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.160 | 2.400 |
Rmerge | 0.072 | 0.055 | 0.243 |
Number of reflections | 20280 | ||
<I/σ(I)> | 9.7 | ||
Completeness [%] | 98.6 | 100 | 87.2 |
Redundancy | 6.4 | 7.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 22% PEG 8K, 120 mM LiSO4, 100 mM Bis Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |