2OFS
Crystal structure of human CD59
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-08 |
Detector | MAR CCD 225 mm |
Wavelength(s) | 1.75 |
Spacegroup name | P 63 |
Unit cell lengths | 56.400, 56.400, 39.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.200 - 2.120 |
R-factor | 0.18 |
Rwork | 0.177 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cdq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.440 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.200 | 2.200 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.051 | 0.141 |
Number of reflections | 4118 | |
<I/σ(I)> | 52.9 | 12.5 |
Completeness [%] | 99.3 | 93 |
Redundancy | 12.2 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 294 | 0.1 M sodium citrate, 16 % polyethylene glycol 6000, 5% isopropanol, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |