2OFS

Crystal structure of human CD59

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-08
Detector	MAR CCD 225 mm
Wavelength(s)	1.75
Spacegroup name	P 63
格子定数 [Å]	56.400, 56.400, 39.400
格子定数 [度]	90.00, 90.00, 120.00

精密化法

残基	28.200 - 2.120
R因子	0.18
Rwork	0.177
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cdq
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.440
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	28.200	2.200
分解能 [Å] (高)	2.120	2.120
Rmerge_l_obs	0.051	0.141
独立反射数	4118
<I/σ(I)>	52.9	12.5
完全性 [%]	99.3	93
冗長性	12.2	10.9

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	3.5	294	0.1 M sodium citrate, 16 % polyethylene glycol 6000, 5% isopropanol, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K