2O34
Crystal structure of protein DVU1097 from Desulfovibrio vulgaris Hildenborough, Pfam DUF375
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-24 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 60.480, 81.480, 151.170 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 19.980 - 1.950 |
| R因子 | 0.213 |
| Rwork | 0.210 |
| R-free | 0.27300 |
| Structure solution method | SAD |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.013 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.483 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 50.000 | 2.070 |
| 分解能 [Å] (高) | 1.980 | 1.980 |
| Rmerge_l_obs | 0.106 | 0.674 |
| 独立反射数 | 55000 | |
| <I/σ(I)> | 8.3 | |
| 完全性 [%] | 96.3 | 63.5 |
| 冗長性 | 4.8 | 3.1 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 4.6 | 295 | 2.0M sodium formate, 0.1M Na acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K, pH 4.60 |
