2JIF
Structure of human short-branched chain acyl-CoA dehydrogenase (ACADSB)
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| 格子定数 [Å] | 150.245, 150.245, 201.586 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 70.000 - 2.000 |
| R因子 | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jqi |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.011 |
| 結合角の平均二乗偏差(RMSD) [度] | 1.287 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 70.360 | 2.110 |
| 分解能 [Å] (高) | 2.000 | 2.000 |
| Rmerge_l_obs | 0.100 | 0.660 |
| 独立反射数 | 175824 | |
| <I/σ(I)> | 12.7 | 2.6 |
| 完全性 [%] | 99.3 | 95.7 |
| 冗長性 | 6.6 | 5.3 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | 6.3 | 30% PEG6000, 0.15M AMMONIUM CHLORIDE PH6.3, 10% ETHYLENE GLYCOL, pH 6.30 |
