2JGD

E. COLI 2-oxoglutarate dehydrogenase (E1o)

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-15
Spacegroup name	P 41 21 2
格子定数 [Å]	142.493, 142.493, 251.811
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	29.090 - 2.600
R因子	0.189
Rwork	0.186
R-free	0.25000
Structure solution method	SAD
結合長の平均二乗偏差(RMSD) [Å]	0.011
結合角の平均二乗偏差(RMSD) [度]	1.079
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	70.000	2.690
分解能 [Å] (高)	2.600	2.600
Rmerge_l_obs	0.140	0.870
独立反射数	80455
<I/σ(I)>	17.2	3
完全性 [%]	99.9	99.8
冗長性	8.7	8.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, SITTING DROP	5.6		SITTING DROP: 1UL 12% PEG 4000, 50MM SOCIUM CITRATE PH5.6. 1UL 10MG/ML TE1O. RESERVOIR: 1UL 12% PEG 4000, 50MM SOCIUM CITRATE PH5.6., pH 5.60