2I3S
Bub3 complex with Bub1 GLEBS motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2005-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97780 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.656, 79.181, 142.582 |
Unit cell angles | 90.00, 98.34, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.221 |
Rwork | 0.219 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u4c |
RMSD bond length | 0.005 |
RMSD bond angle | 1.350 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 79711 | |
Completeness [%] | 90.6 | 68.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 296 | 18% PEG 3K 200 mM NaCl 100 mM Cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |