2HVE
S120G mutant of human nucleoside diphosphate kinase A complexed with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-09-04 |
Detector | MARRESEARCH |
Wavelength(s) | 0.980 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 114.824, 114.824, 89.719 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.402 |
R-factor | 0.22078 |
Rwork | 0.218 |
R-free | 0.27725 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ucn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.256 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.530 |
High resolution limit [Å] | 2.402 | 2.402 |
Number of reflections | 22088 | |
<I/σ(I)> | 21.4 | 4 |
Completeness [%] | 97.1 | 86.7 |
Redundancy | 10.9 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 2.4 M AMMONIUM SULPHATE, 10 MM ADP, 20 MM MGCL2, 4 MM DTT, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |