Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

2HPO

Structure of Aminopeptidase N from E. coli Suggests a Compartmentalized, Gated Active Site

Experimental procedure
Experimental methodMAD
Source typeSYNCHROTRON
Source detailsALS BEAMLINE 8.2.2
Synchrotron siteALS
Beamline8.2.2
Temperature [K]100
Detector technologyCCD
Collection date2005-11-14
DetectorADSC QUANTUM 315
Wavelength(s)0.9797, 0.9798, 0.9612
Spacegroup nameP 31 2 1
Unit cell lengths120.438, 120.438, 170.561
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution19.940 - 1.650
R-factor0.15781
Rwork0.157
R-free0.18062
Structure solution methodMAD
RMSD bond length0.010
RMSD bond angle1.171
Data reduction softwareHKL-2000
Data scaling softwareSCALEPACK
Phasing softwareSHELXS
Refinement softwareREFMAC (5.2.0005)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]20.0001.680
High resolution limit [Å]1.6501.650
Rmerge0.5200.500
Number of reflections164250
<I/σ(I)>14.12
Completeness [%]95.589.2
Redundancy2.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52981.8 M Sodium Malonate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

225158

数据于2024-09-18公开中

PDB statisticsPDBj update infoContact PDBjnumon