2H6V

Spectroscopic and structural study of the heterotropic linkage between halide and proton ion binding to GFP proteins- E2(GFP) APO FORM

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 21 21 21
格子定数 [Å]	51.133, 62.850, 69.631
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	46.620 - 1.470
R因子	0.16017
Rwork	0.159
R-free	0.18692
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.006
結合角の平均二乗偏差(RMSD) [度]	1.350
Data reduction software	MOSFLM
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	46.700	1.540
分解能 [Å] (高)	1.470	1.470
Rmerge_l_obs	0.072	0.156
独立反射数	38294
<I/σ(I)>	5.6	4.1
完全性 [%]	75.0	95
冗長性	7	4.5

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		5		14% (w/v) PEG3350, 100 mM NH4 acetate pH 5.0, 0.2 M NH4F, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 100K