2G18

Crystal Structure of Nostoc sp. 7120 phycocyanobilin:ferredoxin oxidoreductase (PcyA) Apoprotein

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-02
Detector	ADSC QUANTUM 315
Spacegroup name	P 1 21 1
格子定数 [Å]	78.501, 141.332, 121.842
格子定数 [度]	90.00, 91.29, 90.00

精密化法

残基	43.940 - 2.500
R因子	0.21326
Rwork	0.211
R-free	0.25716
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.016
結合角の平均二乗偏差(RMSD) [度]	1.501
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0005)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	43.940	2.590
分解能 [Å] (高)	2.500	2.500
Rmerge_l_obs	0.084	0.383
独立反射数	91664
<I/σ(I)>	13.9	3.3
完全性 [%]	99.7	100
冗長性	3.7	3.6

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1		6.5	293	18% PEG-8000, 25mM MES-NaOH, 50mM Calcium acetate, 10% dioxine, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 6.50