2FQG

Crystal Structures of E. coli Laccase CueO under different copper binding situations

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-12-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
格子定数 [Å]	69.114, 73.036, 189.437
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	50.000 - 2.300
Rwork	0.157
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	1.110
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Quality characteristics

	Overall	Inner shell	Outer shell
分解能 [Å] (低)	50.000	50.000	2.380
分解能 [Å] (高)	2.300	4.950	2.300
Rmerge_l_obs	0.093	0.039	0.463
独立反射数	20806	2286	1956
<I/σ(I)>	7.5
完全性 [%]	95.2
冗長性	3.8	4.1	4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	17% polyethylene glycol 4000, 6% Isopropanol, 100mM tri-Sodium Citrate dihydrate pH 5.6, 10mM CuCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K