2FFQ
The crystal structure of human neuronal Rab6B in its active GTPgS-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9797 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.885, 60.983, 63.685 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.780 |
R-factor | 0.188 |
Rwork | 0.191 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.800 | 1.880 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.052 | 0.228 |
Number of reflections | 13846 | |
<I/σ(I)> | 11.9 | 7.5 |
Completeness [%] | 99.4 | 97.8 |
Redundancy | 6.5 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292.15 | 20% PEG3350, 0.2M ammonium nitrate , pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292.15K |