2EYU
The Crystal Structure of the C-terminal Domain of Aquifex aeolicus PilT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-11 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97934 |
Spacegroup name | P 41 |
Unit cell lengths | 89.150, 89.150, 70.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.870 |
Rwork | 0.170 |
Structure solution method | AB INITIO |
RMSD bond length | 0.007 |
RMSD bond angle | 0.026 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.940 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.080 | 0.197 |
Number of reflections | 44351 | |
<I/σ(I)> | 49.9 | 7.8 |
Completeness [%] | 96.8 | 83.9 |
Redundancy | 12 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 mg/ml protein 0.1 M Mes 10% Dioxane 1.6 M ammonium sulfate , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |