2DLF

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 6.75

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	112
Detector technology	IMAGE PLATE
Collection date	1998-07-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	57.290, 66.930, 53.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.550
R-factor	0.179 *
Rwork	0.184
R-free	0.22800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1igf
RMSD bond length	0.008
RMSD bond angle	27.390 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.520
High resolution limit [Å]	1.550 *	1.550 *
Rmerge	0.052 *	0.318
Total number of observations	331744 *
Number of reflections	29973 *
<I/σ(I)>	42.6	3.5
Completeness [%]	97.7 *	96.3 *
Redundancy	11.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.75	293 *	CRYSTALLIZATION CONDITIONS: TEMPERATURE AT 20C. PRECIPITANT SOLUTION CONTAINED 1 AMMONIUM SULFATE AND 0.025 M SODIUM CACODYLATE (PH 6.75). 5 MG/ML OF FV FRAGMENT., VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	ammonium sulfate	0.8 (M)
3	1	drop	sodium cacodylate	25 (mM)
4	1	reservoir	ammonium sulfate	1.6 (M)
5	1	reservoir	sodium cacodylate	25 (mM)