2DLF

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 6.75

Refinement Statistics

Experimental method:X-RAY DIFFRACTION (1.55 Å)

Cell axes57.29066.93053.630
Cell angles90.0090.0090.00
SpacegroupP 21 21 21
Resolution limits8.00 - 1.55
the highest resolution shell value1.570 - 1.550
R-factor0.184 (0.179*)
R-work0.18400 (0.17900*)
the highest resolution shell value0.340
R-free0.23300 (0.22800*)
the highest resolution shell value0.330
RMSD bond length0.008
RMSD bond angle1.022 (27.390*)

Data Collection Statistics

Resolution limits40.00 - 1.50 (1.55*)
the highest resolution shell value1.57* - 1.55*
Number of reflections32405 (29973*)
Number of measurements331744*
Rmerge_l_obs0.053 (0.052*)
the highest resolution shell value0.318 (0.000*)
Completeness97.6 (97.7*)
the highest resolution shell value96.3*
Redundancy11.1
the highest resolution shell value5.4
I/sigma(I)1

Crystallization Conditions

crystal IDmethodpHpH rangetemperatureunit
1VAPOR DIFFUSION, HANGING DROP6.75293*K*

Crystallization Reagents

IDcrystal IDsolution IDreagent nameconcentrationdetails
Crystallization Reagents in Literatures*
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein5 (mg/ml)
21dropammonium sulfate0.8 (M)
31dropsodium cacodylate25 (mM)
41reservoirammonium sulfate1.6 (M)
51reservoirsodium cacodylate25 (mM)
Annotated Information is extracted from Literature Info*