2DLF

HIGH RESOLUTION CRYSTAL STRUCTURE OF THE FV FRAGMENT FROM AN ANTI-DANSYL SWITCH VARIANT ANTIBODY IGG2A(S) CRYSTALLIZED AT PH 6.75

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	112
Detector technology	IMAGE PLATE
Collection date	1998-07-10
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
格子定数 [Å]	57.290, 66.930, 53.630
格子定数 [度]	90.00, 90.00, 90.00

精密化法

残基	8.000 - 1.550
R因子	0.179 *
Rwork	0.184
R-free	0.22800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1igf
結合長の平均二乗偏差(RMSD) [Å]	0.008
結合角の平均二乗偏差(RMSD) [度]	27.390 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	40.000	1.520
分解能 [Å] (高)	1.550 *	1.550 *
Rmerge_l_obs	0.052 *	0.318
Total number of observations	331744 *
独立反射数	29973 *
<I/σ(I)>	42.6	3.5
完全性 [%]	97.7 *	96.3 *
冗長性	11.1	5.4

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1	VAPOR DIFFUSION, HANGING DROP	6.75	293 *	CRYSTALLIZATION CONDITIONS: TEMPERATURE AT 20C. PRECIPITANT SOLUTION CONTAINED 1 AMMONIUM SULFATE AND 0.025 M SODIUM CACODYLATE (PH 6.75). 5 MG/ML OF FV FRAGMENT., VAPOR DIFFUSION, HANGING DROP

文献の結晶化試薬

ID	結晶ID	溶液	試薬名	濃度 (単位) (単位)	詳細
1	1	drop	protein	5 (mg/ml)
2	1	drop	ammonium sulfate	0.8 (M)
3	1	drop	sodium cacodylate	25 (mM)
4	1	reservoir	ammonium sulfate	1.6 (M)
5	1	reservoir	sodium cacodylate	25 (mM)