2DGA
Crystal structure of hexameric beta-glucosidase in wheat
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2004-12-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 194.650, 194.650, 194.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.210 - 1.800 |
R-factor | 0.19 |
Rwork | 0.189 |
R-free | 0.20200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1v03 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.079 | 0.564 |
Number of reflections | 261540 | |
Completeness [%] | 99.8 | 98 |
Redundancy | 19.5 | 17.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 10mM HEPES pH 7.2, 1M LiSO4, 150mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |