2D00
Subunit F of V-type ATPase/synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-11-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.705, 138.295, 66.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.200 |
Rwork | 0.214 |
R-free | 0.26221 |
Structure solution method | MAD |
RMSD bond length | 0.033 |
RMSD bond angle | 2.492 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 38602 | |
Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 400, calcium acetate, potassium chloride, MES, MOPS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 400, calcium acetate, potassium chloride, MES, MOPS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 400, calcium acetate, potassium chloride, MES, MOPS, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |